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Fig. 3 KD upregulates FGF21 and activates FGF21-KLB signaling in the liver. A Heatmap showing expression of genes related to FGF21 signaling in the liver. Blue, downregulation; red, upregulation. B mRNA expression of Fgf21 in the liver. C FGF21 levels in circulating. D <t>FGFR1</t> and KLB expression in the liver. E, F Immunofluorescence staining of albumin, FGFR1 and KLB in the liver. Green, FGFR1 E and KLB F; blue, DAPI (magnification 200×, scale bar, 100 μm). FGF21, fibroblast growth factor 21; FGFR1, fibroblast growth factor receptor 1; KLB, β-klotho. n = 5 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001.
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Fig. 3 KD upregulates FGF21 and activates FGF21-KLB signaling in the liver. A Heatmap showing expression of genes related to FGF21 signaling in the liver. Blue, downregulation; red, upregulation. B mRNA expression of Fgf21 in the liver. C FGF21 levels in circulating. D <t>FGFR1</t> and KLB expression in the liver. E, F Immunofluorescence staining of albumin, FGFR1 and KLB in the liver. Green, FGFR1 E and KLB F; blue, DAPI (magnification 200×, scale bar, 100 μm). FGF21, fibroblast growth factor 21; FGFR1, fibroblast growth factor receptor 1; KLB, β-klotho. n = 5 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001.
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Fig. 3 KD upregulates FGF21 and activates FGF21-KLB signaling in the liver. A Heatmap showing expression of genes related to FGF21 signaling in the liver. Blue, downregulation; red, upregulation. B mRNA expression of Fgf21 in the liver. C FGF21 levels in circulating. D FGFR1 and KLB expression in the liver. E, F Immunofluorescence staining of albumin, FGFR1 and KLB in the liver. Green, FGFR1 E and KLB F; blue, DAPI (magnification 200×, scale bar, 100 μm). FGF21, fibroblast growth factor 21; FGFR1, fibroblast growth factor receptor 1; KLB, β-klotho. n = 5 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001.

Journal: Nutrition & diabetes

Article Title: Role of liver FGF21-KLB signaling in ketogenic diet-induced amelioration of hepatic steatosis.

doi: 10.1038/s41387-024-00277-3

Figure Lengend Snippet: Fig. 3 KD upregulates FGF21 and activates FGF21-KLB signaling in the liver. A Heatmap showing expression of genes related to FGF21 signaling in the liver. Blue, downregulation; red, upregulation. B mRNA expression of Fgf21 in the liver. C FGF21 levels in circulating. D FGFR1 and KLB expression in the liver. E, F Immunofluorescence staining of albumin, FGFR1 and KLB in the liver. Green, FGFR1 E and KLB F; blue, DAPI (magnification 200×, scale bar, 100 μm). FGF21, fibroblast growth factor 21; FGFR1, fibroblast growth factor receptor 1; KLB, β-klotho. n = 5 mice per group. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Fibroblast growth factor receptor 1 (FGFR1) (60325-1-Ig) antibody was purchased from Proteintech (Wuhan, China).

Techniques: Expressing, Staining